Evaluation of combinations of low hazard biocides in controlling mold and stain fungi on southern pine

Two laboratory methods and a field test were used in this study. An initial lab test was used to rapidly screen a large number of potential biocides. The formulations that were most effective in the rapid screening method were then tested by a final laboratory procedure designed to simulate the severity of field conditions. In this method, the treated specimens were sprayed with a suspension containing macerated mycelium of a variety of mold and stain fungi and block-stacked. The formulations used in the field test were based on the results of the severe lab test. The treated test specimens were incubated in the summer under natural conditions, rated after 4 and 8 weeks, and compared to specimens treated with a commercial Na Pentachlorophenoxide (Na PCP)/borax formulation. After 4 weeks of field exposure, 0.20 percent copper-8-hydroxyquinolinolate (Cu-8) plus 0.50 percent Busan 1009 and 0.02 percent Cu-8 plus 0.5 percent Busan 110 controlled fungal growth as well as 2.0 percent Na PCP plus 3.2 percent borax; 0.01 percent Cu-8 plus 0.20 percent Skane M-8 performed better than 1.0 percent Na PCP plus 1.6 percent borax. None of the low-hazard biocide combinations controlled fungal growth adequately after 8 weeks.

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