Experimental Investigation of Gluing and Finishing Problems Through Application of Fluorescence Microscopy in Incident Light

A simple technique was developed for microscopic visual and photographic observations of glued-wood joints and finishes. The procedure consists of staining both the wood and the adhesive or finish with contrasting fluorescent stains. These stains emit visible light upon incidence of ultra-violet light. The visible portion of the UV spectrum is filtered out at the source. All the remaining UV light that is not absorbed by the specimen is filtered in the ocular of the microscope. Specimens were prepared by soaking, surfacing with a sliding type microtome, immersing in the dye solution, washing in water, then completely saturating with water through vacuum soaking. The specimen remains in water, covered by glass during observation. Studies showed that soaking in Rhodamin3 GD at 1:100 concentration for a period of 1 hour followed by a 15 minute wash is preferred for wood staining. Urea-formaldehyde resin adhesive is stained with Pyranin at 1:100, in an amount of 10 percent on the resin dispersion ready for use.

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